〔Abstract〕 Objective To establish a normal reference interval for antiphospholipid antibody (aPL) levels in healthy pregnant women of childbearing age, and explore the changes in aPL levels at different gestational weeks. Methods A total of 200 healthy pregnant women of childbearing age who visited the Sixth People's Hospital of Nanhai District, Foshan City from January 2023 to December 2023 were selected. Chemiluminescence immunoassay (CLIA) was used to detect the levels of immunoglobulin (Ig) A, IgM and IgG of anti-cardiolipin antibody (aCL), anti-β2-glycoprotein Ⅰ antibody (aβ2GPI) at different gestational weeks, and the percentile values were calculated by non-parametric method. Results In healthy pregnant women of childbearing age, the levels of aCL IgA, IgM, IgG, and aβ2GPI IgA, IgM, IgG exhibited a skewed distribution (P < 0.001). Comparison of the levels of aCL IgA,IgM, IgG, and aβ2GPI IgA, IgM, IgG in pregnant women at different gestational weeks showed no statistically significant differences (P > 0.05). The reference intervals for aCL IgA, IgM, IgG, and aβ2GPI IgA, IgM, IgG in the serum of healthy pregnant women of childbearing age were as follows: the P99 values were 8.49 PL–U·mL^-1, 12.58 PL–U·mL^-1, 5.68 PL–U·mL^-1, 20.17 RU·mL^-1, 36.79 RU·mL^-1, and 8.37 RU·mL^-1, respectively, and the P95 values were 5.16 PL–U·mL^-1, 6.10 PL–U·mL^-1, 3.39 PL–U·mL^-1, 17.48 RU·mL^-1, 25.60 RU·mL^-1, and 6.53 RU·mL^-1, respectively. Conclusion The levels of aCL and aβ2GPI in the bodies of pregnant women at different gestational weeks are relatively stable. This study has preliminarily established the reference intervals for aCL and aβ2GPI, which can provide some reference for the screening and diagnosis of APS.