〔Abstract〕 Objective To explore the changes of magnetic resonance imaging (MRI) signals of tendon healing within 1 year after anterior cruciate ligament reconstruction (ACLR), and to explore the relationship between tendon healing and clinical subjective function score. Methods The signal changes of tendine-bone junction on MRI at different time nodes at 3, 6, and 12 months after ACLR with hamstring tendon were performed in 30 male patients in Shenzhen Second People's Hospital from January 2019 to December 2020 were retrospectively analyzed. The healing degree of tendon bone was indicated by the signal-to-noise ratio (SNQ) of the intraosseous grafts of tibia and femur. Repeated measurement ANOVA was used to compare the differences in SNQ values of intraosseous grafts at different time nodes, and T-test was used to compare the differences in SNQ values of intraosseous grafts of tibia and femur at the same time nodes. Results At 3, 6, and 12 months after operation, SNQ values of femoral and tibial grafts gradually increased at 3 months, reached a peak at 6 months, and then gradually decreased. The SNQ values of the grafts in the femoral segment were significantly different at 3, 6, and 12 months (F = 6.88, P < 0.05). There was no significant difference in SNQ value of tibial segment grafts among different time nodes at 3, 6, and 12 months (F = 2.87, P >0.05). Within 1 year after surgery, the SNQ value of femoral segment graft was significantly higher than that of tibia segment at the same time point, the difference was statistically significant (P < 0.05). Conclusion The signal of anterior cruciate ligament tendine-bone junction reached a peak in June, and then gradually decreased, and the healing of femur segment was worse than that of tibia segment.