〔Abstract〕 Objective To explore the effects of medium frequency pulse acupoint electrical stimulation combined with rehabilitation nursing on cervical function and pain in patients with neck, shoulder, waist and leg pain. Methods 114 patients with neck, shoulder, waist and leg pain admitted to Zhengzhou Neck, Shoulder, Waist and Leg Pain Hospital from January 2021 to May 2022 were selected and divided into a control group and an observation group by random number table method, with 57 cases in each group. The control group was given routine treatment and nursing, and the observation group was given medium frequency pulse acupoint electrical stimulation combined with rehabilitation nursing on the basis of the control group. The treatment effectiveness, visual analogue scale (VAS) score, serum pain factor level, neck, shoulder, waist and leg function and daily living ability of the two groups were observed. Results There was no significant difference in VAS score, pain attack frequency, pain attack duration, serum pain factor level, neck, shoulder, waist and leg function score and Oswestry dability index (ODI) between the two groups before intervention (P > 0.05). After intervention, the total treatment response rate, clinical assessment scale for cervical spondylosis (CASCS) score, Constant-Murley shoulder joint function score, Japanese Orthopaedic Association scores (JOA) and Lysholm knee joint function score in the observation group were significantly higher than those in the control group, VAS score, pain attack frequency, pain attack duration, substance P (SP), norepinephrine (NE) and 5-hydroxytryptamin (5-HT) levels and ODI score were lower than those of the control group, and the differences were statistically significant (P < 0.05). Conclusion Medium frequency pulse acupoint electrical stimulation combined with rehabilitation nursing can effectively reduce the level of serum pain factors in patients with neck, shoulder, waist and leg pain, relieve pain symptoms, improve the function of neck, shoulder, waist and leg and the ability of daily life.