〔Abstract〕 Objective To analyze the clinical application effect of precision platelet-rich plasma combined with traditional Chinese medicine ironing in the treatment of knee osteoarthritis. Methods A total of 60 patients with mild to moderate knee osteoarthritis admitted to Traditional Chinese Medicine Hospital of Yulin from July 2023 to December 2023 were selected and divided into an observation group (treated with precision platelet-rich plasma combined with traditional Chinese medicine ironing) and a control group (treated with sodium hyaluronate combined with traditional Chinese medicine ironing), with 30 cases in each group. The efficacy of the two groups was compared. Results After treatment, the visual analogue scale (VAS) score of the observation group was lower than that of the control group, and the Lysholm scale score was higher than that of the control group, with statistically significant differences (P < 0.05). After treatment, the knee joint range of motion (ROM) and activity of daily living (ADL) scores of the observation group were higher than those of the control group, with statistically significant differences (P < 0.05). After treatment, the Western Ontario and Mcmaster Universities osteoarthritis index (WOMAC) of the observation group was lower than that of the control group, with a statistically significant difference (P < 0.05). The total effective rate of the observation group was higher than that of the control group, with a statistically significant difference (P < 0.05). The overall satisfaction of the observation group was higher than that of the control group, with a statistically significant difference (P < 0.05). Conclusion When conditions permit, the use of precision platelet-rich plasma combined with traditional Chinese medicine for the treatment of knee osteoarthritis can achieve better clinical outcomes, significantly reducing pain and restoring patients’ ability to perform daily activities.