〔Abstract〕 Objective To explore the influence of the construction of regional collaborative rescue system of chest pain center on the treatment of acute ST-segment elevation myocardial infarction (STEMI) in Zhengzhou. Methods The STEMI referral patients admitted before and after the establishment of the chest pain center of Zhengzhou Central Hospital from October 2015 to October 2020 were divided into routine referral group and regional collaborative referral group, with 90 cases in each group. Both groups were treated with percutaneous coronary intervention (PCI) after admission. The total ischemic time after onset, symptom oneset to first medical contact (S2FMC), first medical contact-to-wire (FMC2W), and the incidence of clinical adverse events were compared between the two groups. Results The total ischemic time, S2FMC, FMC2W in the regional collaborative referral group were shorter than those in the routine referral group, the differences were statistically significant (P < 0.05). The incidence of clinical adverse events in the regional collaborative referral group was lower than that in the routine referral group, the difference was statistically significant (P < 0.05). Conclusion The construction of regional collaborative rescue system in chest pain center significantly improves the clinical rescue efficiency of STEMI referral patients, reduces the incidence of clinical adverse events, and improves the prognosis of patients.