〔Abstract〕 Objective To analyze the infection status of chlamydia trachomatis (CT), ureaplasma urealyticum (UU) and neisseria gonococcus (NG) in female urogenital tract infections in Zhengzhou in 2022. Methods Urogenital tract secretions of 626 female patients with urogenital tract infection admitted to Henan Provincial People's Hospital from January 2022 to December 2022 were collected, and CT, UU and NG were detected by polymerase chain reaction (PCR) + membrane hybridization method, and the infection distribution of CT, UU and NG were analyzed. Results Among the 626 patients, the total number of CT, UU and NG positive cases was 386 (61.66%), including 21 CT infections (3.35%), 383 UU infections (61.18%) and 4 NG infections (0.64%). There were 365 cases of single pathogen infection (58.31%) and 21 cases of mixed infection (3.35%). The mixed infection rate was lower than that of single pathogen infection, and the mixed infection was most common with CT+UU infection, the difference was statistically significant (P < 0.05). Among them, the infection rates of CT, UU and NG were the highest in patients aged 18-34 years, and the positive rates of CT, UU and NG infection were the lowest in patients aged ≥ 60 years. Conclusion In 2022, CT, UU and NG infections were the main types of female urogenital tract infections in Zhengzhou, among which UU infection was at high risk, and CT+UU infection was more common in mixed infections. Young and middle-aged people were in high-risk groups.