〔Abstract〕 Objective To explore the clinical efficacy of Shenmai Injection intervention in the treatment of cancer-related fatigue in gastric cancer with Qi and Yin deficiency type. Methods Sixty six patients with Qi and Yin deficiency type gastric cancer induced fatigue admitted to the Department of Spleen and Stomach Diseases and Oncology at Jingjiang Traditional Chinese Medicine Hospital from September 2020 to October 2022 were selected as the study subjects. They were randomly and parallelly divided into a control group and an observation group, with 33 cases in each group. During the research process, 6 cases were dropped, and the last 60 cases entered statistical analysis, with 30 cases in each group. The control group patients received routine symptomatic treatment, while the observation group patients received an additional injection of Shenmai Injection on the basis of routine symptomatic treatment. Both groups of patients were treated for 14 days. The effects before and after treatment, Piper fatigue scale (PFS) score, Karnofsky performance status (KPS) score, traditional Chinese medicine syndrome score and adverse reactions were compared between the two groups. Results After treatment, the PFS score and traditional Chinese medicine syndrome score of the observation group were lower than those of the control group, and the KPS score was higher than that of the control group, with statistical significance (P < 0.05); The total effective rate of the observation group was 73.33%, which was higher than 46.67% of the control group's, and the difference was statistically significant (P < 0.05); Both groups of patients did not experience any adverse drug reactions during treatment. Conclusion Shenmai Injection has a good therapeutic effect and good safety in the treatment of gastric cancer-related fatigue with Qi and Yin deficiency.