〔Abstract〕 Plant-derived exosome-like nanoparticles are bilayer-membranous vesicles isolated from plant tissues with a diameter of about 40 ~ 300 nm. They are secreted by living cells. They are important carriers of biological information between cells and play an important role in communication between cells. In order to further study the bio-logical mechanism of exosomes, the extraction of high-quality exosomes is the first step. In this paper, the exosomes of Epimedium were extracted by ultra-fast centrifugation method and purified by sucrose heavy water pad method, and the microscopic identification of exosomes was consistent with that of exosomes. We hoped that this paper can provide some references for researchers to explore exosome extraction of Chinese herbal medicine.