〔Abstract〕 Objective To compare the effects of different luteal support schemes in fresh cycle transplantation with antagonist for ovulation promotion. Methods A total of 80 infertility patients who underwent fresh cycle transplantation with antagonist regimen to promote ovulation in Xuzhou Maternal and Child Health Hospital from January 2019 to March 2021 were selected as the study objects. Clinical data of the patients were retrospectively analyzed, and they were divided into an observation group and a control group, with 40 cases in each group, according to different luteal support programs. The control group was given progesterone + estrogen + gonadotropin-releasing hormone analogue (GnRHa) regimen for luteal support. The observation group was given progesterone + estrogen + GnRHa + human chorionic gonadotropin (HCG) regimen for luteal support. Ovulation induction, embryo development and clinical outcome were compared between the two groups. Results There was no significant difference in ovulation promotion and embryo development between the two groups (P > 0.05). The clinical pregnancy rate and live birth rate of the observation group were higher than those of the control group, while the ectopic pregnancy rate and abortion rate were lower than those of the control group, the differences were statistically significant (P < 0.05). There was no significant difference in the multiple birth rate between the two groups (P > 0.05). Conclusion Progesterone + estrogen + GnRHa + HCG regimen for luteal support after fresh cycle transplantation with antagonist stimulating ovulation can significantly improve the clinical pregnancy rate and live birth rate of infertility patients, and improve the pregnancy outcome of patients, which is an ideal luteal support regimen for fresh cycle transplantation with antagonist stimulating ovulation.