〔Abstract〕 Objective To explore and analysis the distribution characteristics of microorganisms in cervical secretions of female patients with urogenital infections. Methods A total of 345 cases of female patients who were admitted to Jiaozuo Second People's Hospital for gynecological hospitalization from September 2019 to December 2022 were selected as the research subjects. The cervical secretions from patients were extracted, microbial distribution was detected, and multivariate analysis was conducted. At the same time, drug sensitivity tests were performed on specific microorganisms. Results There were 86 cases diagnosed as urinary and reproductive tract infections in the 345 cases that accounted for 24.93%. A total of 75 strains of pathogenic bacteria were isolated, including 40 strains of Gram negative bacteria, 30 strains of Gram positive bacteria, and 5 strains of fungi. The top three ranked bacteria in distribution were Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus. In the 22 strains of Escherichia coli, the resistance rate to amikacin were 0.00%, while the resistance rates to ceftriaxone, cefazolin, cefuroxime and chloramphenicol were all lower than 20.00%. The resistance rate to ampicillin were the highest, reaching 90.91%. The binary logistic regression analysis showed that diabetes, length of stay in hospital, smoking and age were the important factors leading to the occurrence of genitourinary tract infection (P < 0.05). Conclusion The bacteria in cervical secretion of female patients with genitourinary tract infection are mainly Gram negative bacteria, and their drug resistance is relatively low. Diabetes, hospital stay, smoking and age are important factors leading to female genitourinary tract infection.