〔Abstract〕 Objective To analyze the effects of Xuebijing injection on vital signs, blood gas indexes and levels of inflammatory factors in patients with sepsis complicated with acute lung injury. Methods A total of 127 patients with sepsis complicated with acute lung injury admitted to Zhengzhou Orthopaedic Hospital from April 2020 to April 2023 were selected and divided into a control group (63 cases) and an observation group (64 cases) by random number table method. The control group was given routine treatment, and the observation group was combined with Xuebijing injection on the basis of the control group. After 1 week of treatment, the vital signs, blood gas indexes, levels of inflammatory factors and occurrence of adverse reactions were compared between the two groups. Results After 1 week of treatment, the heart rate and body temperature of the observation group were lower than those of the control group, and the systolic blood pressure and diastolic blood pressure were higher than those of the control group, the differences were statistically significant (P < 0.05). After 1 week of treatment, the arterial partial pressure of oxygen (PaO2) and arterial oxygen saturation (SpO2) in the observation group were higher than those in the control group, and the arterial partial pressure of carbon dioxide (PaCO2) was lower than that in the control group, with statistical significances (P < 0.05). After 1 week of treatment, the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) in the observation group were lower than those in the control group, and the differences were statistically significant (P < 0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P > 0.05). Conclusion Xuebijing injection can effectively improve blood gas indexes, stabilize vital signs, and reduce the level of inflammatory factors in patients with joint sepsis complicated with acute lung injury, with high safety.