〔Abstract〕 Objective To study the pharmacological bioactive substances and mechanism of ZaoZaoShui(ZZS) with main tonifying plant drinks. Methods The plant drinks ZZS was entrusted to Shenzhen Academy of Metrology and Quality Inspection, which had been certified by multiple domestic and foreign authoritative institutions. Experimental testing was conducted in accordance with relevant national standards and Technical Specifications for Inspection and Evaluation of Health Food (2003 edition). Results The plant drinks ZZS contained a variety of amino acids, calcium, vitamin B6, flavonoids, saponins and so on. Conclusion Based on the theory of insomnia with multiple deficiencies, one of the possible neuropharmacological mechanisms of the plant drinks ZZS with tonifying Qi-blood, strengthening spleen and nourishing liver and kidney, and nourishing heart and calming mind were achieved by maintaining the normal metabolism of neurons, regulating the central nervous system and related neurotransmitters, sedating hypnosis, neuroprotection and other nervous system functions.