〔Abstract〕 Objective To investigate the drug resistance and distribution characteristics of clinically isolated carbapenemresistant Enterobacteriaceae bacteria (CRE), and their carrying integrons and carbapenem genes. Methods A total of 659 nonrepetitive CRE strains were collected from inpatients in Zhengdong Branch of the First Affiliated Hospital of Zhengzhou University from January 2021 to December 2021. The VITEK 2-compact automatic microbial identification and drug sensitivity analysis system was used for bacterial identification and drug sensitivity testing. The susceptibility tests for ceftriaxone, cefuroxime, and ampicillin/ sulbactam were performed using the paper diffusion method. Carbapenem genes and class I integrase genes were detected by the polymerase chain reaction (PCR) method. Results 659 strains of CRE were mainly distributed in the intensive care unit (45.22 %), respiratory intensive care unit (13.96 %), emergency intensive care unit (9.26 %), urology department (7.74 %), and respiratory department (4.10 %). The resistance rates to β-lactam drugs and quinolone drugs were more than 90 %. The detection rates of blaKPC, bla_NDM, and bla_IMP genes were 81.33 %, 10.93 %, and 0.30 %, respectively. 5 strains of carbapenem-resistant Klebsiella pneumoniae (CRKP) carried bla_KPC and blaIMP genes at the same time. The positive rates of class I integrase in CRKP and carbapenem resistant Escherichia coli (CREC) coli were 90.94 % and 74.07 %, respectively. Conclusion Among 659 strains of CRE, CRKP has a high isolation rate,the drug-resistant genes are mainly bla_KPC and bla_NDM, and the detection rate of class I integron is high. The prevention and control measures of nosocomial infection should be strengthened to effectively control the spread of this kind of bacteria.