〔Abstract〕 Objective To explore the relationship between blood glucose and colpitis mycotica in elderly female patients with diabetes mellitus. Methods A total of 80 elderly women with diabetes admitted to Pingdingshan Maternal and Child Health Care Hospital from May 2019 to May 2022 and 80 elderly women who underwent physical examination in Pingdingshan Maternal and Child Health Care Hospital during the same period were selected as the research objects. The 80 elderly female patients with diabetes were divided into diabetes group and the 80 patients who underwent physical examination were divided into physical examination group. The incidence of mycotic vaginitis was compared between the two groups. The incidence of mycotic vaginitis in diabetic patients with good and poor blood glucose control was compared. To compare the recurrence rate of mycotic vaginitis in diabetic patients with good and poor glycemic control. Results The incidence of mycotic vaginitis in the diabetes group was higher than that in the physical examination group, and the difference was statistically significant (P < 0.05). The incidence of mycotic vaginitis in patients with poor blood glucose control was higher than that in patients with good blood glucose control, and the difference was statistically significant (P < 0.05). The recurrence rate of mycotic vaginitis in patients with poor blood glucose control was higher than that in patients with good blood glucose control, the difference was statistically significant (P < 0.05). Conclusion The blood glucose level of elderly female diabetic patients is closely related to the incidence of mycotic vaginitis. The higher the blood glucose level, the higher the incidence and recurrence rate of mycotic vaginitis.