〔Abstract〕 Objective To investigate the effects of acupuncture combined with conventional rehabilitation therapy on motor function, general function and quality of daily life in patients with post-stroke shoulder-hand syndrome (SHS). Methods A total of 178 patients with post-stroke SHS admitted to the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine from March 2021 to March 2022 were selected for a prospective study and randomly divided into a control group and an observation group, with 89 cases in each group. Patients in both groups were given basic treatment such as lowering blood pressure, anti-platelet aggregation and nutritional support, and rehabilitation training of good limb position, passive and active exercise, and acupuncture therapy was added to patients in the observation group. The therapeutic effect of the two groups was compared. Results After 1 month of treatment, the total effective rate of pain improvement in the observation group was 92.13 %, which was higher than 68.53% in the control group, and the difference was statistically significant (P < 0.05). The total effective rate of upper limb motor function improvement in the observation group was 82.02 %, which was higher than 66.29 % in the control group, and the difference was statistically significant (P < 0.05). Visual analog scale (VAS) scores of the two groups were decreased to varying degrees after treatment, the scores of Fugl-Meyer assessment (FMA), comprehensive assessment of physical function (bFCA), and quality of life of the two groups were improved to varying degrees, and the VAS score of the observation group was lower than that of the control group after treatment.The scores of FMA, bFCA and quality of life were higher than those of the control group, and the differences were statistically significant (P < 0.05). Conclusion Acupuncture combined with conventional rehabilitation therapy in the treatment of post-stroke SHS can effectively improve the motor function, comprehensive function and daily life quality of patients, and the efficacy is better than rehabilitation therapy alone.