〔Abstract〕 Objective To study the predictive value of combined detection of peripheral blood D-dimer, B-type natriuretic peptide (BNP) and procalcitonin (PCT) in lower extremity deep vein thrombosis in long-term bedridden patients. Methods A total of 80 long-term bedridden patients diagnosed and treated in Jiaozuo People's Hospital from January 2022 to June 2022 were selected as the research objects. According to whether the patients had lower extremity deep venous thrombosis, they were divided into lower extremity deep venous thrombosis group (an observation group) and no lower extremity deep venous thrombosis group (a control group). The differences in D-dimer, BNP and PCT were detected and compared between the two groups. Logistic regression analysis of influencing factors of lower extremity deep venous thrombosis in long-term bedridden patients. To analyze the sensitivity and specificity of combined detection of peripheral blood D-dimer, BNP and PCT in predicting lower extremity deep venous thrombosis in long-term bedridden patients. Results The levels of D-dimer, BNP, and PCT in the observation group were significantly higher than those in the control group, and the differences were statistically significant (all P < 0.05). Logistic regression analysis showed that D-dimer, BNP and PCT were influencing factors of lower extremity deep venous thrombosis in long-term bedridden patients (P < 0.05). The sensitivities of D-dimer, BNP, and PCT in predicting long-term bed rest combined with lower extremity deep venous thrombosis were 0.975, 0.900, and 0.950, and the specificities were 1.000, 0.975, and 1.000, respectively. The cut-off values of D-dimer, BNP, and PCT in predicting long-term bed rest combined with lower extremity deep venous thrombosis were 5.46 mg·L-1, 111.87 pg·mL-1, and 3.25 mg·L-1, respectively. Conclusion The combined detection of D-dimer, BNP and PCT can provide reference for the prediction of deep venous thrombosis of lower limbs in long-term bedridden patients.