〔Abstract〕 Objective To investigate the effect of inactivation of virus in blood at 56 °C for 30 min on the results of indicators of pre-transfusion infectivity by light initiated chemilu minescent assay (LiCA). Methods Two tubes of blood from 20 patients were collected, and after inactivation at 56 °C for 30 min, the transmissibility indexes [hepatitis C virus antibody (HCV-Ab), human immunodeficiency virus antibody (HIV-Ab), treponema pallidum antibody (TP-Ab), hepatitis B surface antigen (HBsAg), hepatitis B surface antibody (HBsAb), hepatitis B e antigen (HBeAg), hepatitis B e antibody (HBeAb) and hepatitis B core antibody (HBcAb)] were detection, and comparison of the detection results of the indicators before and after inactivation and the difference of the signal value of the luminous assay. Results Before and after inactivation at 56 °C for 30 min, there was no statistically significant difference in the signal value of the luminescence measurement of all the infectious indicators before blood transfusion (P > 0.05). The coincidence rate of qualitative analysis before and after inactivation of all indicators was 100 %. Pearson correlation analysis showed that before and after inactivation, the infective indexes before and after transfusion were highly correlated (r > 0.7, P < 0.01). Conclusion The inactivation treatment of blood at 56 °C for 30 min will not affect the results of LiCA detection of indicators of pre-transfusion infectivity. This inactivation method can be used as a sample pretreatment method in highly infectious virology laboratories.