〔Abstract〕 Objective To explore the disinfection effect of the new portable chlorine dioxide vaporization disinfection device compared with the conventional ozone disinfection of bed unit in infant incubator terminal disinfection. Methods A total of 100 incubators were selected from the neonatal department of Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine from August 2021 to September 2021, and were randomly divided into an observation group and a control group, with 50 incubators in each. The control group was treated with conventional ozone disinfection, and the observation group was treated with portable chlorine dioxide vaporization. The disinfection effects of the two methods were compared. Results The qualified rate of air bacteria detection in the observation group was 98.00 %, higher than 84.00 % in the control group, and the difference was statistically significant (P < 0.05). The qualified rate of bacteria detection on object surface in the observation group was 100.00 %, higher than 88.00 % in the control group, and the difference was statistically significant (P < 0.05). The detection rate of airborne bacteria in the observation group was 34.00 %, lower than 90.00 % in the control group, and the difference was statistically significant (P < 0.05). The detection rate of bacteria on the surface of objects in the observation group was 42.00 %, lower than 86.00 % in the control group, and the difference was statistically significant (P < 0.05). Conclusion Compared with the conventional ozone disinfection method, the new convenient chlorine dioxide vaporizing disinfection device used in the final disinfection of infant incubator has the advantages of better disinfection effect and portability.