〔Abstract〕 Objective To explore the effect evaluation of 3D printing individualized knee joint prosthesis in animal experiments. Methods A total of 10 Zijin Lantang pigs from Guangdong Heyuan were selected as experimental pigs for computed tomography (CT) image acquisition of knee joint. The obtmined image date were imported into Mimics 17.0 software, and the software was used to repair and erase the sectional image, and personalized knee joint prosthesis was designed. Left total knee arthroplasty (TKA) was performed on experimental pigs, and 3D printing knee prostheses were implanted. The knee extension and flexion of experimental pigs were observed, the pain and muscle strength of the left hindlimb were evaluated, and imaging examination was performed to observe the joint space, prosthesis loosening, wound and prosthesis infection. Results The operation was carried out smoothly, and in 3 months after the operation, the left hind limb pain, muscle strength and activity were good. Anteroposternal and lateral radiographs and CT examination results of the knee joint, anatomical observation of the knee prosthesis complex, indicating that the position of the joint prosthesis, lower limb length, line of force, joint space is good, no obvious loosening and infection. Conclusion The application of 3D printing individual knee prosthesis in animal experiment of TKA is good and feasible.