〔Abstract〕 Objective To analyze the effect of sufentanil combined with propofol on respiration and circulation in children during clinical anesthesia. Methods A total of 80 children who needed clinical anesthesia and were treated in the Second Affiliated Hospital of Nanhua University from July 2020 to December 2021 were selected and divided into a control group and an observation group by odd and even number grouping, with 40 cases in each group. The control group was treated with propofol alone, and the observation group was treated with sufentanil on the basis of the control group. The anesthetic effects of the two groups were compared. Results The blood oxygen saturation and airway pressure of the observation group were higher than those of the control group, and the end-tidal partial pressure of carbon dioxide and heart rate were lower than those of the control group, and the differences were statistically significant (P < 0.05). The anesthesia recovery time, anesthesia onset time, anesthesia induction time and extubation time of the observation group were shorter than those of the control group, and the differences were statistically significant (P < 0.05). The excellent rate of anesthesia in the observation group was higher than that in the control group, the difference was statistically significant (P < 0.05). The incidence of adverse reactions in the observation group was lower than that in the control group, and the difference was statistically significant (P < 0.05). Conclusion In the development of pediatric clinical anesthesia, the anesthetic effect of sufentanil combined with propofol is better, which can have a small impact on the respiration and circulation of children, with a low incidence of adverse reactions and high safety.