〔Abstract〕 Objective To explore the clinical value of high-risk human papilloma virus (HPV) deoxyribonucleic acid (DNA) typing combined with thinprep cytologic test (TCT) and colposcopy in the early screening of cervical precancerous lesions. Methods Affiliated Hospital of Sun Yat-Sen University from January 2019 to January 2022 were retrospectively analyzed, and both groups of patients were examined by high-risk HPV-DNA typing, TCT and colposcopy. The pathological examination was used as the gold standard to analyze the diagnostic efficiency of high-risk HPV-DNA typing, TCT and colposcopy and the combination detection, and the differences were compared. Results The sensitivity, specificity and accuracy rate of high-risk HPV-DNA typing + TCT + colposcopy in the diagnosis of cervical precancerous lesions were 98.00 % (98/100), 38.33 % (23/60) and 75.62 % (121/160) respectively; The sensitivity of high-risk HPV-DNA typing + TCT + colposcopy were higher than those of high-risk HPV-DNA typing, TCT and colposcopy. The sensitivity of TCT + colposcopy, high risk HPV-DNA typing + colposcopy, high risk HPV-DNA typing + TCT, colposcopy, high risk HPV-DNA typing sensitivity were higher than those of TCT single test; The sensitivity of high risk HPV-DNA typing + colposcopy and high risk HPV-DNA typing +TCT were higher than those of colposcopy, and the differences were statistically significant (P < 0.05). The accuracy of high risk HPV-DNA typing + TCT + colposcopy, TCT + colposcopy, high risk HPV-DNA typing + colposcopy, high risk HPV-DNA typing +TCT, colposcopy, high risk HPV-DNA typing were higher than those of TCT single examination, and the differences were statistically significant (P < 0.05). There was no statistical significance in the accuracy of other test schemes (P > 0.05). There was no statistical significance in the specificity, positive predictive value and negative predictive value among different test schemes (P > 0.05). Conclusion In the early screening of cervical precancerous lesions, the combined examination of high-risk HPV-DNA typing, TCT and colposcopy can significantly improve the sensitivity and reduce the missed diagnosis compared with the single examination.