〔Abstract〕 Objective To investigate the relationship between epstein-barr virus (EB) infection and nasal and nasopharyngeal diseases. MethodsThe data of electronic nasopharyngoscopy in Guangzhou Cadre Health Management Center from September 2019 to December 2020 were collected. The results of electronic nasopharyngoscopy EB virus detection of all the research subjects were statistically analyzed, and the incidence of different EB virus infection by nasopharyngoscopy was compared. Results Among 711 subjects, there were 234 cases of EB virus viral capsid antigen (VCA)-immunoglobulin A(IgA) antibody positive and 477 cases of EB virus VCA-IgA antibody negative. There were 93 cases positive for EB virus nuclear antigen 1 (NA1) -IgA antibody and 618 cases negative. There were 23 cases with both positive and 407 cases with both negative results. Among the abnormal results of nasopharyngeal endoscopy, nasopharyngitis had the highest incidence. The incidence of nasopharyngeal carcinoma was significantly higher in patients with EB virus NA1-IgA antibody positive than that in patients with EB virus NA1-IgA antibody negative. The incidence of nasal polyps was significantly higher in patients with EB virus VCA-IgA antibody negative than that in patients with EB virus VCA-IgA antibody positive than that in patients with EB virus VCA-IgA antibody positive. The differences were all statistically significant (P < 0.05). Conclusion EB virus has a certain correlation with the occurrence of nasal and nasopharyngeal related diseases. Patients with positive EB virus VCA-IgA antibody and NA1-IgA antibody have a higher incidence of nasopharyngitis, which may be involved in the occurrence of nasal polyps and nasopharyngeal carcinoma respectively, but further studies are needed.