〔Abstract〕 Objective To explore the clinical efficacy of Bifidobacterium combined with acupuncture at Tianshu acupoint in the treatment of chronic constipation. Methods A total of 102 patients with chronic constipation admitted to Fujian Funeng Group General Hospital from May 2019 to May 2021 were randomly divided into a control group and an observation group, with 51 cases in each group. The control group was treated with bifidobacterium, and the patients in the observation group were treated with acupuncture at Tianshu acupoint on the basis of the control group. The therapeutic effects of the two groups were observed and compared. Results The total effective rate of the observation group was higher than that of the control group, the symptom severity score in the observation group were lower than those in the control group after treatment, the dimension score and total score of patient-assessment of constipation quality of life (PAC-QOL) of the observation group after treatment were lower than those of the control group, and the differences were statistically significant (P < 0.05). There was no statistically significant difference in the total incidence of adverse reactions between the two groups (P > 0.05). Conclusion Bifidobacterium combined with acupuncture at Tianshu acupoint is effective in the treatment of chronic constipation, which can effectively improve the symptoms of constipation and improve the quality of life, with high safety.