〔Abstract〕 Objective To observe the examination status of thyroid nodules among medical workers Shenzhen Second People's Hospital, and analyze the related influencing factors. Methods The physical examination data of medical workers in Shenzhen Second People's Hospital of Shenzhen from January 2020 to December 2020 were retrospectively analyzed. A total of 749 medical workers with complete physical examination data were included. The correlation between ultrasound examination of thyroid nodules and thyroid function, blood glucose, blood lipid, blood pressure, body mass index (BMI), job title and other factors was statistically analyzed. Results The ultrasonic detection rate of thyroid nodules in staff was 43.66 % in Shenzhen Second People's Hospital. The detection rate of thyroid nodules in female was higher than that in male, and the detection rate of senior title was higher than that of junior or intermediate title. The thyroid nodules detection rate of 45-55 years old group was higher than that of < 35 years old group, and the thyroid nodules detection rate of > 55 years old group was higher than that of < 35 years old group and 35-45 years old group, respectively, the differences were statistically significant (P < 0.05). Multivariate logistic regression analysis showed that age, diastolic blood pressure (DBP), BMI and total cholesterol (TC) were independent risk factors for thyroid nodules (P < 0.05). Conclusion The detection rate of thyroid nodules in medical workers in the Second People's Hospital of Shenzhen was correlated with age, blood lipid, blood pressure and BMI. The detection rate of thyroid nodules in senior job title was higher, which may be related to the more mental and work stress. Therefore, it is necessary to develop appropriate follow-up plans for medical workers at different levels, and give life guidance and stress relief.