〔Abstract〕 Objective In order to prevent ureteral injury in complicated gynecological surgery, to explore the clinical value of ureteral intubation before surgery. Methods The screening period of the research subjects was from May 2021 to Aprial 2022, and a total of 21 patients who had undergone complex surgery in the gynecology department of General Hospital of the Third Division of Xinjiang Production and Construction Corps during the one-year period were screened, including 14 patients with cervical cancer, 1 patient with adenomyosis, 1 patient with giant broad ligament, 1 patient with cervical fibroids, 2 patients with giant ovarian tumor, and 3 patients with endometriosis. All patients were inserted into the ureteral catheter (D-J tube if necessary) before the surgery, and then the corresponding surgical treatment was carried out. After the surgery, the ureteral catheter was removed and the catheter was indwelled. Results The 21 patients successfully completed the corresponding surgical treatment, the average time of ureteral catheterization was (8.5 ± 2.9) min, and the average surgery time was (89.7 ± 15.2) min. No intraoperative complications occurred in all patients, and 3 patients developed hematuria after surgery, and all recovered spontaneously within 3 days after surgery. There was no ureteral infection or pain symptoms within 10 days after surgery, and no hydronephrosis and ureteral dilatation were found in the ultrasound examination. Conclusion For patients who need complex gynecological surgery, preoperative ureteral intubation under the guidance of cystoscope can help clinicians quickly identify and separate the ureter during the surgery, effectively avoid intraoperative ureteral injury, and the intubation method is quick and safe.