〔Abstract〕 Objective To investigate the efficacy of T cell spot test for tuberculosis infection (T-SPOT.TB), sputum phage combined with purified protein derivative of tuberculin (PPD) test in the diagnosis of tuberculosis. Methods A total of 82 suspected tuberculosis patients admitted to the General Hospital of Yima Coal Industry Group Co. LTD from January 2018 to December 2020 were selected as the study subjects. All patients received T-SPOT.TB, sputum phage and PPD detection. The diagnostic results of the three methods were counted, and the sensitivity, accuracy and specificity were calculated and compared. Results The sensitivity and accuracy of T-SPOT.TB combined with the three were higher than those of the sputum phage and PPD, and the differences were statistically significant (P < 0.05). There was no statistical significance in the sensitivity and accuracy of the combined detection of the three components compared with T-SPOT.TB (P > 0.05), and there was no statistical significance in the sensitivity and accuracy of sputum phage and PPD (P > 0.05). ConclusionThe diagnostic value of the combined detection of the three methods was better than the independent detection of sputum phage, PPD and T-SPOT.TB.