〔Abstract〕 Objective Comparing the perceptual eye positions of adolescents with different degrees of myopia can provide more basis for visual function for the prevention and control of myopia. Methods Randomly selected myopia patients from the ophthalmology clinic of Shenzhen Kuiyong People's Hospital of Dapeng New District from July to August 2020 as the research subjects. The patients were divided into three groups according to the difference of spherical equivalent: mild myopia group (sphere lens -0.50 ~ -3.00 D), moderate myopia group (sphere lens -3.25 ~ -6.00 D) and high myopia group (spherical lens ~ -6.00 D), a detailed ophthalmological examination including perceptual eye position, uncorrected visual acuity and best-corrected visual acuity, slit lamp examination, fundus examination, intraocular pressure, apparent and dilated refraction, and eyeballs were performed on all subjects sports. Results A total of 209 patients were included, including 116 cases in mild myopia group, 51 cases in moderate myopia group, and 42 cases in the high myopia group. The quartiles of horizontal perception eye position pixels are as follows: mild myopia group: 8 (2.25, 20.75); moderate myopia group: 12.00 (4.00, 66.00); high myopia group: 52 (20.75, 72.25); There was no difference in perceived eye position between mild myopia and moderate myopia (adjusted P = 0.128). The difference of perceived eye position between mild myopia and high myopia was statistically significant (adjusted P < 0.05). There was statistically significant difference in horizontal perception between moderate myopia and high myopia (adjusted P < 0.05). The quartiles of vertical perception eye position pixels were as follows: 3.00 (2.00, 5.00) in the mild myopia group, 4.00 (2.00, 6.00) in the moderate myopia group, and 4.00 (2.00, 8.25) in the high myopia group; There was no significant difference in vertical perceptual eye position among the three groups of myopia (P > 0.05). Conclusion In patients with mild myopia, moderate myopia and high myopia, there were obvious deviations in the horizontal perception eye position, indicating that the horizontal perception eye position pixels may be related to the degree of myopia.