〔Abstract〕 Objective To investigate the effect of Chaiqin Qingning capsules combined with oseltamivir granules on acute upper respiratory tract infection caused by influenza B virus infection. Methods A total of 90 patients with acute upper respiratory tract infection admitted to Beijing Aerospace General Hospital from January 2021 to January 2022 were selected. Randomly divided into an observation group and a control group, with 45 cases in each group. Chaiqin Qingning capsules combined with oseltamivir granules and oseltamivir granules were given for the two groups respectively. The curative effect and relief time of symptoms (high fever, sore throat, stuffy nose and fatigue) were compared between the two groups, and changes of C-reactive protein (CRP), procalcitonin (PCT) and quality of life assessment scale (SF-36) before and after treatment were recorded, as well as the incidence of adverse reactions during treatment. Results The total effective rate of the observation group was 97.78%, higher than 84.44% of the control group, the difference was statistically significant (P < 0.05). The relief time of fatigue, high fever, nasal congestion, sore throat and other symptoms in the observation group were shorter than those in the control group, the differences were statistically significant (P < 0.05). After treatment, the levels of CRP and PCT in the two groups were lower than those before treatment, and the levels of CRP and PCT in the observation group were lower than those in the control group, the differences were statistically significant (P < 0.05). After treatment, the scores of physical function, material life, emotional function and social function in the two groups were higher than those before treatment, and the scores of the physical function, material life, emotional function and social function in the observation group were higher than those in the control group, with statistical significances (P < 0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P > 0.05). Conclusion Chaiqin Qingning capsules combined with oseltamivir granules are more effective in the treatment of acute upper respiratory tract infection caused by influenza B virus infection, and they can quickly relieve symptoms, inhibit the expression of inflammatory factors and improve the quality of life of patients, and less adverse reactions safe and effective