〔Abstract〕 Objective To study the effect of premature rupture of membranes combined with reproductive tract infection on pregnancy outcome of pregnant women. Methods 60 cases of pregnant women with premature rupture of membranes complicated with reproductive tract infection admitted to Zhuhai People's Hospital from January 2021 to December 2021 were selected as the observation group, and another 60 healthy pregnant women examined in Zhuhai People's Hospital during the same period were selected as the control group. The two groups of pregnant women were followed up, and compared the delivery methods, pregnancy outcomes and neonatal outcomes of the two groups. Results The rate of natural delivery and vaginal delivery in the observation group was lower than that in the control group, and the cesarean delivery rate was higher than that in the control group, with statistical significances (P < 0.05). The incidence of placental abruption, postpartum hemorrhage, puerperal infection, chorioamnitis and other adverse pregnancy outcomes in the observation group were higher than those in the control group, with statistical significances (P < 0.05). The incidence of premature birth, neonatal infection rate, neonatal asphyxia and neonatal respiratory distress syndrome in the observation group were higher than those in the control group, and the differences were statistically significant (P < 0.05). Conclusion Premature rupture of membranes combined with reproductive tract infection will have adverse effects on the delivery mode and maternal and infant outcomes. Therefore, positive treatment measures and nursing intervention should be taken for such pregnant women in clinical practice to reduce the risk of adverse pregnancy outcomes and effectively protect maternal and infant health.