〔Abstract〕 Objective To investigate the clinical application of Pilates exercise combined with biomimetic electrical stimulation in the treatment of postpartum rectus muscle separation. Methods A total of 60 patients with postpartum rectus abdominis separations admitted to Nansha Hospital of the First People's Hospital of Guangzhou from September 2019 to September 2020 were selected and divided into control group and observation group by random number table method, with 30 patients in each group. The control group received conventional bioelectric stimulation treatment, and the observation group received Pilates exercise combined with bioelectric stimulation treatment. The clinical efficacy of the two groups were recorded. The separation distance of rectus abdominis muscle, the improvement of low back pain of patient satisfaction before and after treatment were observed in the two groups. Results After treatment, the separation width of the superior rectus abdominis muscle and the separation distance between the inferior rectus abdominis muscle in the two groups were shortened to varying degrees, and the separation width of the superior rectus abdominis muscle and the separation distance between the inferior rectus abdominis muscle in the observation group were shorter than those in the control group, with statistical significances (P < 0.05). After treatment, the proportion of lumbago and back pain in both groups decreased to varying degrees, and the proportion of lumbago and back pain in the observation group was lower than that in the control group, the difference was statistically significant (P < 0.05). The satisfaction of the observation group was 93.33%, which was higher than 73.33% of the control group, and the difference was statistically significant (P < 0.05). Conclusion Pilates exercise combined with bioelectric stimulation can improve the overall therapeutic effect of patients with postpartum rectus abdominis muscle separation, effectively reduce the degree of low back pain, improve the separation distance of rectus abdominis muscle and waist circumference recovery, and increase the overall satisfaction.