〔Abstract〕 Objective To analyze the effect of pre-stored autologous blood transfusion used in obstetrics Rh(D) negative blood transfusion. Methods 100 pregnant women in Sanming First Hospital from March 2019 to March 2021 adopt pre-stored autologous blood transfusion used in obstetrics Rh(D) negative blood transfusion. To compared the changes of hemoglobin (Hb), red blood cells deposited (HCT) and platelet (PLT) levels before and after blood collection and transfusion, and statistics on the mode of delivery and neonatal outcomes were analyzed. Results All the 100 pregnant women completed pre-stored autologous blood transfusion collection. The vital signs of mother and infants were stable and no blood donation reaction occurred during blood collection. 67 pregnant women received autologous blood transfusion, and there were no adverse reactions. There was no significant difference in Hb, HCT and PLT levels before and after blood collection (P > 0.05). Among the 100 pregnant women, 72 cases delivered naturally and 28 cases delivered by cesarean section. The neonatal body weight was 2.75 ~ 4.13 kg, with an average of (3.34 ± 2.25) kg, and Apgar score was 9 ~ 10 points, with an average of (9.53 ± 0.15) points. Conclusion Pre-stored autologous blood transfusion in the treatment of obstetrics Rh(D) negative blood transfusion has a good effect. The vital signs of pregnant women and fetuses are stable before and after blood collection and transfusion, and there is no blood donation reaction, and the newborn is in good condition.