〔Abstract〕Objective To explore the application effect of time-interval appointment and partition management mode in virus nucleic acid sampling in general outpatient clinics of designated hospitals for COVID-19. Methods A total of 1773 patients with viral nucleic acid sampling who make an appointment to the general outpatient department of the Third People's Hospital of Shenzhen from April 11 to April 24, 2020 were selected as the observation objects. The time-segment appointment and zoning management mode was not implemented from April 11 to 17, which was set as "before implementation". The time-segment reservation and partition management mode was implemented from April 18 to 24, which was set as "after implementation" to compare the number of samples and the situation of personnel gathering before and after implementation. The epidemiological data and psychological state of anxiety and depression of 200 patients were investigated by using simple random sampling method and self-made questionnaire, and the psychological state of anxiety and depression of different genders were compared. Results 780 patients were sampled before the implementation of time-segment appointment and partition management; After execution, 993 patients were sampled, more than before; After execution, 774 samples were collected from sampling area 1 and 219 samples were collected from sampling area 2, the differences were statistically significant (P < 0.05). In the 7 days after the time-segment reservation, the average number of samples from 8:00 to 9:00 and from 9:00 to 11:00 were more than those from the first 7 days, the differences were statistically significant (P < 0.05). In the sample survey of 200 patients, anxiety and depression accounted for 62.00 %, and the proportion of obvious feeling "nervous" reached 47.00 %. The proportion of anxiety and depression in male was 65.91%, higher than 54.41% in the female, the differences were statistically significant (P < 0.05). Conclusion In the nucleic acid sampling of COVID-19 virus, the management mode of time-segment appointment and partition can effectively reduce the clustering of patients and their families and prevent crossinfection. At the same time, nurses have targeted psychological counseling for patients with anxiety, which can effectively relieve patients' psychological pressure and negative emotions of anxiety and depression.