〔Abstract〕Objective To establish a microbial limit test method for Compound snake-ant spray. Methods The compound the compound snake-ant spray by Huangpu People's Hospital of Zhongshan from November 2017 to December 2017 was selected. The recovery ratio of the test bacteria was as an index. The total number of aerobic bacteria, fungus and yeast were counted by plate method and membrane filtration method. The effective detection of staphylococcus aureus and pseudomonas aeruginosa in the tested samples was taken as the index, and the control bacteria were examined by plate method and membrane filtration method. Results When the count method of aerobic bacteria was membrane filtration method and the count method of fungus and yeast was plate method, the ratio of colony number of experimental groups to that of liquid control group was between 0.5 and 2.0. Control bacteria inspection method using membrane filtration method, can detect staphylococcus aureus, pseudomonas aeruginosa. Conclusion The method established in this study is suitable for the microbial limit test of compound snake-ant spray.