〔Abstract〕Objective To study and analyze the clinical effect of Shenqi Fuzheng injection combined with radiotherapy in the treatment of advanced lung cancer. Methods 80 patients with advanced lung cancer admitted to the First Affiliated Hospital of Henan University from January 2020 to January 2021 were selected by random sample sampling method, and divided into control group and observation group according to the time of admission, with 40 cases in each group. The control group was treated with radiotherapy, and the observation group was treated with radiotherapy combined with Shenqi Fuzheng injection. The therapeutic effect, serum related indicators, interleukin-2 (IL-2) level, natural killer cells (NK), lymphokine-activated killer cells (LAK) and adverse reactions of the two groups were compared. Results The total effective rate of the observation group was 95.00%, which was higher than 70.00% of the control group, the difference was statistically significant (P < 0.05). After treatment, the levels of matrix metalloproteinase-9 (MMP-9) and basic fibroblast growth factor (bFGF) in the observation group were significantly lower than those in the control group, the differences were statistically significant (P < 0.05). After treatment, the levels of IL-2, NK and LAK cells in the observation group were significantly higher than those in the control group, the differences were statistically significant (P < 0.05). The incidence of adverse reactions was 2.50%, lower than 20.00% in the control group, the difference was statistically significant (P < 0.05). Conclusion Shenqi Fuzheng injection combined with radiotherapy has significant effect on advanced lung cancer.