〔Abstract〕 Objective To explore the application value of human papillomavirus (HPV) E6/E7 in the diagnosis of cervical cancer. Methods A total of 100 subjects who underwent cervical lesion screening in Jieyang Maternal and Child Health Care Family Planning Service Center from January 2019 to December 2019 were selected as the research objects. All subjects were detected using HPV DNA and HPV E6/E7 messenger RNA (mRNA). The diagnostic efficacy of the two detection methods and the diagnostic results at different ages were compared using colposcopic histopathology as the gold standard and the diagnostic results of the two tests were compared at different ages.. Results The diagnostic sensitivity, specificity and positive predictive value of HPV E6/E7 mRNA were higher than those of HPV DNA, and the differences were statistically significant (P ＜ 0.05). Under the two detection methods, the positive detection rates of patients aged 36-55 were higher than those of other age groups, with statistical significance (P ＜ 0.05). Conclusion The sensitivity, specificity and positive predictive value of HPV E6/E7 mRNA detection for cervical cancer are higher than that of HPV DNA detection. In addition to colposcopy tissue examination, HPV E6/E7 mRNA and HPV DNA clinical diagnosis results, a comprehensive judgment should also be made based on the patient's age to avoid missed diagnosis in the clinical diagnosis of cerviccal cancer