〔Abstract〕 Objective To explore the influence of plasma preparation time and quick freezing method on cryoprecipitate quality. Methods 120 whole blood donors collected by Ningde Central Blood Station from June 2020 to December 2020 were selected. They were divided into two groups by random number table method including group A and group B, with 60 cases in each group.The preparation time was 6h, 8h, 12h and 18h, respectively, with 15 cases in each group at different preparation time. Group A was quick-frozen by plasma freezer, group B was quick-frozen by low-temperature storage box, and the coagulation factor VIII (FVIII) and fibrinogen content in two groups were compared under different plasma preparation time and different quick-frozen methods. Results There were statistically significant differences in FVIII activity between groups A and B under different preparation time (P < 0.05); The FVIII activity of group B under different preparation time was lower than that of group A, with statistical significance (P < 0.05). There was no statistical significance in the fibrinogen content of group A and group B under different preparation time (P > 0.05); There was no significant difference in fibrinogen content between the two groups under different preparation time (P > 0.05). Conclusion Different plasma preparation time has a certain effect on the quality of cryoprecipitate. The longer the plasma preparation time, the lower the content of cryoprecipitate coagulation factor. The effect of cryoprecipitate prepared by plasma quick-freezing method is better than that by low-temperature storage box.