〔Abstract〕 Objective To investigate the distribution and change of hepatitis C virus (HCV) genotype in patients with chronic hepatitis C (CHC) in Henan province, and to analyze the factors related to the epidemic characteristics of HCV genotype. Methods HCV genotyping was performed by Core and/or NS5B sequencing phylogenetic tree, univariate and multivariate methodsto analyze the factors related to HCV genotype distribution. Results Seven genotypes were detected in this study: 1b (58.6%), 2a (15.7%), 6a (13.8%), 3a (7.5%), 3b (2.8%), 1a (0.8%), 2b (0.8%). 356 patients were included in the statistical analysis. Logistic regression model showed that type 1b was associated with the infection route of blood transfusion or blood products (OR = 2.22, P = 0.009), and type 6a was associated with the infection route of drug use (OR = 3.18, P = 0.009). Type 1b was more common in patients over 40 years old (OR = 1.02, P = 0.023), while type 6a was more common in patients over 40 years old and below (OR = 0.97, P = 0.029). Conclusion The change of HCV genotype distribution reflects the shift of transmission route, and currently, HCV type 1b is mostly found in patients over 40 years old in Henan, while type 6a has an increasing trend and is mostly found in patients 40 years old and younger.