〔Abstract〕 Objective To establish a method for the determination of phenol in calamine zinc oxide lotion. Methods High performance liquid chromatography (HPLC) was used, the chromatographic column was Welch Xtimate? C18 (5 μm, 4.6 mm×150 mm), methanol-water (40:60) was used as the mobile phase for isocratic elution, the flow rate was 1.0 mL·min-1, column temperature was 30 °C and injection volume was 10 μL. The detection wavelength was 270 nm. Results Phenol had a good linear relationship in the range of 62.08 ~ 434.56 μg·mL-1 (r > 0.999), the sample recovery rate was 100.46%, and the relative standard deviation (RSD) was 0.28%. Conclusion The method established in this study is simple, fast, accurate, reproducible, and highly precise. It can be used for the quality control of phenol in calamine zinc oxide lotion.