〔Abstract〕 Objective To prepare and validate a bimodal nanoprobe for glioma. Methods A polymerization method was used to combine indocyanine green (ICG), DOTA-Gd, and Angiopep-2 to form a bimodal contrast agent. Results The complexes were stable without precipitation, and the surface zeta potential of the BSA-ICG-DOTA-Gd complex showed 10.9 mV, while the results of multifunctional enzyme standardization showed that the complexes had ICG fluorescence properties, with excitation by light at 700 nm and emission at 820 nm. peak at 820 nm. The UV absorption test of the complex showed a clear protein absorption peak at 280 nm, and the BSA-ICG-DOTA-Gd complex showed a clear fluorescence under the excitation of 720 nm wavelength light by the small animal fluorescence imaging system, and the results of animal experiments showed that the fluorescence could reach the peak by injecting the complex ICG-DOTA-Gd-ANG2 for 0.5 h, and then declined immediately. Conclusion This dual-mode contrast agent has good stability and fluorescence, and has potential applications.