〔Abstract〕 Objective To evaluate the diagnostic value of anti-Mullerian hormone (AMH) and sex hormone in ovarian reserve of infertile women and analyze the related factors. Methods 43 infertile women diagnosed in the Fourth Department of Gynecology, Fujian Maternal and Child Health Hospital affiliated to Fujian Medical University from May 2018 to September 2020 were included. Six basic sex hormones were measured on the third day of the menstrual cycle, and AMH was measured on any day of the menstrual cycle. According to ovarian function ,they were divided into decreased ovarian reserve (DOR) group and normal ovarian reserve (NOR) group. The levels of six sex hormones and AMH were compared between the two groups. Receiver operating Characteristic Curve (ROC) was used to compare the predictive value of AMH and sex hormones in evaluating ovarian reserve function, and the correlation between AMH and related variables was analyzed. Results The level of AMH in DOR group was significantly lower than that in NOR group, and the levels of estradiol (E2) and testosterone (T) in DOR group were significantly higher than that in NOR group, with statistical significance (P < 0.05). Among the single indexes, E2 had the largest area under ROC curve (AUC), with a cut-off value of 49.00 pg·mL-1. The AUC of combined diagnosis was 0.945, with a sensitivity of 94.70% and specificity of 87.50%.AMH was negatively correlated with age and follicle stimulating hormone (FSH). Conclusion AMH has the stability of menstruation, and the detection is basically not affected by other factors, while sex hormones are easily affected by menstrual cycle and other feedback regulation. AMH and sex hormones can be used to jointly evaluate ovarian reserve function of infertile women in clinical practice to increase its accuracy.