〔Abstract〕 Objective To investigate the curative effect of cervical ligation in the treatment of cervical insufficiency in the second trimester. Methods A total of 82 parturitions with cervical insufficiency admitted to Huizhou First Maternal and Child Health Hospital from June 2018 to May 2021 were selected and divided into control group and observation group according to random number table method, with 41 cases in each group. Control group received dydrogesterone tablets treatment, and observation group received cervical ligation treatment.The neonatal survival rate, body quality, newborn asphyxia rate ,other neonatal prognosis and gestational age, term birth, abortion, premature delivery were compared between the two groups. The parturients in the observation group were divided into group A (21 cases, operation at 14-16 weeks of gestation) and group B (20 cases, operation at 16-24 weeks gestation) according to different operation periods. The pregnancy outcome were compared in different operation periods. Results The survival rate and birth weight of neonates in the observation group were higher than those in the control group, and the neonatal asphyxia rate was lower than that in the control group, the differences were statistically significant (P < 0.05). The gestational age and full term birth rate in the observation group were higher than those in the control group, and the abortion rate and premature birth rate were lower than those in the control group, the differences were statistically significant (P < 0.05). There was no significant difference in maternal abortion rate between group A and group B (P > 0.05). The full-term birth rate and neonatal survival rate in group A were higher than those in group B, and the preterm birth rate was lower than that in group B, the differences were statistically significant (P < 0.05). Conclusion Cervical ligation can prolong the gestational age of parturient women with cervical insufficiency, improve the neonatal prognosis and pregnancy outcome, and cervical ligation at 14 to 16 weeks of gestation is the best time for operation.