〔Abstract〕 Objective To investigate and analyze the effects of dexmedetomidine on intraoperative hemodynamics and stress response during hysterectomy under general anesthesia. Methods A total of 305 patients with hysterectomy under general anesthesia admitted to Pingmei Shenma Medical Group General Hospital from September 1, 2018 to September 1, 2019 were analyzed. All patients were divided into control group with 152 cases and observation group with 153 cases by random number method. The control group was given 0.9% sodium chloride injection, the observation group was given dexmedetomidine, and the specific situation of the two group were observed and analyzed. Results There was no statistical significance in hemodynamic indexes before anesthesia induction between two groups (P > 0.05). The heart rate (HR), mean arterial pressure (MAP) and systolic blood pressure (SBP) of the observation group immediately after operation were lower than those of the control group, the differences were statistically significant (P < 0.05). The levels of cortisol (Cor), interleukin-6 (IL-6) and norepinephrine (NE) in the observation group were lower than those of the control group, and the differences were statistically significant (P < 0.05). Conclusion Dexmedetomidine can effectively improve the hemodynamics indexes of patients and reduce stress response during hysterectomy under general anesthesia.