〔Abstract〕 Objective To investigate the distribution characteristics and trend changes of human immunodeficiency virus (HIV) infection among unpaid blood donors in Shunde area, so as to provide data basis for the adjustment and development of blood donor recruitment strategies and improve the blood quality and safety level. Methods 235,416 unpaid blood donors in Shunde area from January 2014 to December 2020 were selected for HIV screening. Enzyme-linked immunosorbent assay (ELISA) and nucleic acid testing (NAT) were used for testing. Any responsive blood samples from either of the two methods should be sent to Shunde District Center for Disease Control and Prevention for confirmation by western blotting. Results Among 235,416 specimens, 28 were confirmed positive for HIV, with a positive rate of 11.89/100,000 (28/235,416)．There were 27 cases of male and 1 case of female. The infection rate of male (17.14/100,000) was higher than that of female (1.28/100,000), showing a statistical difference.The infection rate of first-time blood donors (17.99/100,000) was higher than that of repeated blood donors (8.19/100,000), with statistical difference(P < 0.05). From January 2014 to December 2020, the proportion of homosexual (male to male) transmission showed an overall increasing trend, with an proportion of 46.43% (13/28). Conclusion There is a certain proportion of HIV infection among unpaid blood donors in Shunde area.The positive rates of male blood donors, first-time blood donors are relatively higher. Ensure the safety of blood transfusion, it is suggested that blood stations should recruit unpaid blood donors from low-risk groups based on local conditions when formulating blood donation recruitment strategies and promoting knowledge of unpaid blood donation.