〔Abstract〕 Objective To study and analyze the diagnostic effect of serum procalcitonin (PCT), interleukin-6 (IL-6) and C-reactive protein (CRP) levels in leukemia patients with chemotherapy complicated with sepsis. Methods 68 patients with leukemia chemotherapy admitted to Qingyang People's Hospital from January 2020 to June 2021 were selected. 38 patients complicated with sepsis were selected as the observation group, and 30 patients without sepsis were divided into the control group. All patients underwent enzyme-linked immunoassay. The levels of serum PCT, IL-6 and CRP in the two groups were observed, and diagnostic effects of each indicator detection and combined detection were analyzed. Results After examination, the levels of serum PCT, IL-6 and CRP in the observation group were significantly higher than those in the control group, and the differences were statistically significant (P < 0.05). The sensitivity of combined detection of PCT, IL-6 and CRP was significantly higher than that of single detection (P < 0.05). Conclusion Combined detection of serum PCT, IL-6 and CRP has high diagnostic value in the detection of chemotherapy complicated with sepsis in leukemia patients.