〔Abstract〕 Objective Analyze the level of anti-Miller hormone and sex hormone to evaluate the clinical value of ovarian reserve. Methods From May 2019 to July 2020, 400 women of childbearing age who underwent pre-pregnancy examination in Luoding People’s Hospital were selected and divided into five groups according to age difference. The anti-Miller tube hormone levels of selected subjects were measured by electrochemiluminescence. As well as luteinizing hormone, progesterone, testosterone, follicleforming hormone, estradiol, and prolactin were tested, and the test results of each group were compared and analyzed. Results With the increase of age, the level of anti-Müllerian hormones in women of childbearing age gradually decreases, and the differenced of anti-Müllerian hormone levels between different ages was statistically significant (P < 0.05); Except for estradiol and progesterone , comparison of sex hormone levels between different ages, the difference was statistically significant (P < 0.05). Conclusion For women of childbearing age, testing the levels of anti-millerian hormones and sex hormones can effectively evaluate ovarian reserve.