〔Abstract〕 Objective To investigate the effect of miR-612 on gastric cancer cells by inhibiting CD151 gene overexpression invasion and migration. Methods Human gastric cancer cell line SGC-7901 was cultured and transfected with miR-612 mimicry (miR-612 group) or pcDNA3.1 - CD151 overexpression vector (CD151 group). Then we detected the expression of miR-612 and CD151 mRNA in SGC-7901 cells by real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR). Proliferation, apoptosis, invasion and migration of SGC-7901 cells were detected by MTT method, flow cytometry and transwell chamber, respectively. Results Compared with the miR-NC group, the expression level of miR-612 in miR-612 group was significantly increased, while the expression level of CD151 mRNA was significantly decreased (P < 0.05). Compared with CD151 group, the proliferation activity of miR-612 +CD151 group was significantly decreased after transfection 72 h (P < 0.05). The apoptosis rate of miR-612 +CD151 group was significantly higher than that of CD151 group (P < 0.05). Compared with the control group, the number of invading cells and migrating cells in Mir-612 group were decreased, while the number of invading cells and migrating cells in CD151 group increased significantly (P < 0.05). The number of invading cells and migrating cells in Mir-612+CD151 group were decreased (P < 0.05). Conclusion miR-612 can inhibit the proliferation, invasion, migration of SGC-7901 cells and promote apoptosis of gastric cancer cells by targeting CD151 expression.