〔Abstract〕 Objective To explore the value of local anesthesia with lidocaine for patients who need rectal prostate biopsy in clinic. Methods From April 2018 to April 2020, 33 patients who underwent rectal prostate biopsy in Huidong County People's Hospital were randomly divided into three groups: Group A (2% lidocaine), Group B (0.9% sodium chloride injection) and Group C (blank group), with 11 patients in each group. The pain and complications of the three groups were compared. Results The painless rate of patients in group B was significantly higher than that in group C, and the painless rat of patients in group A was significantly higher than that in groups B and group C, and the difference was statistically significant (P < 0.05). The incidence of complications in group B was significantly lower than that in group C, and the incidence of complications in group A was significantly lower than that in group B and group C (P < 0.05). Conclusion Local anesthesia with lidocaine is not only safe, but also effective in relieving pain with few complications.