〔Abstract〕Objective To observe the effects of sequential therapy of estradiol valerate combined with progesterone on hormone levels, anemia and endometrial thickness in patients with adolescent dysfunctional uterine bleeding. Methods A total of 74 adolescent patients with dysfunctional uterine bleeding treated in the First Affiliated Hospital of Xinxiang Medical College from May 2018 to January 2020 were selected and divided into combined group (37 cases) and conventional group (37 cases) according to random number table method. The combined group received sequential estradiol valerate combined with progesterone, while the conventional group received estradiol valerate alone for 3 menstrual cycles. The differences of bleeding control time and complete hemostasis time between 2 groups were observed, serum sex hormones (LH, FSH and E2) were recorded before treatment and 3 menstrual cycles after treatment, and the incidence of adverse drug reactions in two groups during treatment was statistically analyzed. Results The bleeding control time and complete hemostasis time in combined group were significantly shorter than those in conventional group, with statistical significance (P < 0.05). After 3 menstrual cycles, the levels of LH, FSH and E2 in two groups were significantly lower than before, and the levels of LH, FSH and E2 the combined group was significantly lower than those in the conventional group, the differences were statistically significant (P < 0.05). During treatment, there was no significant difference in the incidence of adverse drug reactions such as nausea, vomiting, headache and breast tenderness between two groups (P > 0.05). Conclusion Estradiol valerate sequentially combined with progesterone has a good effect on the treatment of adolescent dysfunctional uterine bleeding, which can obviously improve the anemia of patients, adjust the endocrine environment of the body, reduce endometrial thickness, and is beneficial to the recovery of prognosis.