〔Abstract〕 Objective To compare the clinical effect of point–of–care testing (POCT) and automatic biochemical analyzer in blood glucose detection. Methods A total of 100 diabetic patients and healthy subjects admitted to Jiangmen Xinhui District People's Hospital from March 2018 to March 2020 were selected. Venous whole blood samples with different glucose concentrations were obtained, and 4 patients ≤ 2.8 mmol·L-1 were included in group 1. Sixteen patients with the range of 2.8 ~ < 4.2 mmol·L-1 were included in group 2. Twenty patients with the range of 4.2 ~ < 6.7 mmol·L-1 were included in group 3. Thirty patients ranging from 6.7 to 11.1 mmol·L-1 were included in group 4. 14 ranged from 11.1 to 16.6 mmol·L-1 and were included in group 5; Eight patients with the range of 16.6 ~ 22.2 mmol·L-1 were included in group 6. Eight patients > 22.2 mmol·L-1 were included in group 7. 42 POCT glucose meters and automatic biochemical analyzer were used to detect blood glucose in each group, and the detection effects of the two instruments were compared and analyzed. Results The test results showed that there was no significant difference between the test resultsof POCT and automatic biochemical analyzer in each group (P > 0.05). The linear regression analysis showed that the correlation coefficient r value of group 4 and group 5 was the highest, r value was 0.999 ~ 0.997, and the linear regression equation was y = 1.0075x + 0.1752. All the test resultswere consistent with the relevant criteria in “The Guideline for Blood Glucose Measurement by Portable Glucose Meter”. The results of the automatic biochemical analyzer and 42 POCT glucose meters were all within the permitted range, that is, all the glucose meters involved in the comparison were qualified. Conclusion In blood glucose detection, POCT blood glucose meter has no significant difference with fully automated biochemical analyzer. POCT blood glucose meter has simple operation, good stability and repeatability.