〔Abstract〕 Objective To explore the value of modified Carba NP method and carbapenemase inactivation (CIM) method forrapid detection of carbapenem-resistant antibacterial drugs in Acinetobacter baumannii and Pseudomonas aeruginosa. Methods The 88 strains of Pseudomonas aeruginosa and 88 strains of Acinetobacter baumannii clinically isolated from Dongguan Chang'an Hospital from March 2020 to March 2021 were included as the research objects. All the strains used the modified Carba NP method and CIM method After detection, genetic testing was used as the gold standard to observe the detection value of the two methods (sensitivity, specificity, negative predictive value and positive predictive value). Results The detection sensitivity (96.15%) and negative predictive value (75.00%) of Pseudomonas aeruginosa by the CIM method were higher than those of the modified Carba NP method (84.62%, 29.41%), and the differences were statistically significant (P < 0.05) ; Compared with the specificity (90.00%), positive predictive value (98.68%) and the modified Carba NP method (50.00%, 92.96%), the difference was not statistically significant (P > 0.05); acinetobacter baumannii was detected by the CIM method Sensitivity (90.67%), specificity (92.31%), negative predictive value (63.16%) and (98.55%) are all higher than the modified Carba NP method (78.67%, 53.85%, 30.43%, 90.77%), and the differences are statistically significant Academic significance (P < 0.05). Conclusion The CIM method is more valuable than the modified Carba NP method for the rapid detection of carbapenem-resistant antibacterial drugs in Acinetobacter baumannii and Pseudomonas aeruginosa.